The present study was carried out to investigate the estrous behavior and vaginal smear after induction of estrus with exogenous hormones in the premature, metestrous and anestrous bitches.
A total of 21 bitches(Mixed breed: 16, Jindo breed: 5), from 10 months to 5 years of age and weighing 8 to 15 ㎏ was studied the change of vaginal smear and the estrous behavior before and after induction of estrus.
The results obtained are as follows:
1. In the treatment A(They were given the PGF₂α, estrone, estradiol-17β, PMSG and HCG) proestrus commenced in 10.16±.44(Mean±SEM) days after treatment, The mean duration of proestrus, and estrus was 7, 50±1. 44 and 13. 50±3. 44 days, respectively.
In the treatment B(They were given the PMSG and HCG) proestrus commenced in 5.53±0.59 days after treatment, The mean duration of proestrus and estrus was 6. 60±0. 71 and 14. 60±1.14 days, respectively.
2. All of the 6 bitches in the treatment A showed vulval swelling and vaginal discharge, 14 of the 15 bitches in the treatment B showed vulval swelling and vaginal discharge. However, all of the treatment A and B showed male acceptance,
3. The main change of vaginal smear in proestrus and estrus after induction of estrus was a increase in the proportion of anuclear and superficial cells associated with a decrease in small intermediate and parabasal cells, By the estrous behavior and vaginal smear the estrus was induced in all the premature, metestrous and anestrous bitches.
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